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Hi, I am trying to calibrate the spectrometer but I am running in some trouble. I have the HG-2 calibration lamp from Ocean Insight. But when I try and calibrate the spectrum received only shows limited spectral lines. Is there any possibility to manually set the wavelength of specific spikes? The software does not handle the big gap between the spikes.

Kind regards

Hello Dave,

Up to now I have tested only with NE-2 lamp. Would you be kind enough to send a screenshot on what does the HG-2 lamp spectrum looks like ?

Hi

Attached should be the emission lines from the light source, yet the other image is what is measured on the sensor. Through some analysis, the leftmost emission line should be the one at 546.074nm as this can be filtered away by the dichroic lens. Is there any way to manually set the emission lines on dedicated emission lines?

It's indeed not ideal but you should be able to perform a linear model at least.

What happens when you do that ? Can you send a screenshot ?

You can also try with more directive settings like:

wavelength range: 500 - 700 nm
span: 100-120 nm
distortion: 0-10 nm

that should force the model to the proper initial settings

I think I finally got to a point where I calibrated my spectrometer, now for a primary test with Ethanol I do get a result as follows. This does not look promising if I compare it against the data from the performance page of the starter edition on the website. Any idea why it is so different?

Could you try with acetone instead ? If you used denaturated ethanol (the blue stuff) you may get plenty of fluorescence signal from the additives.

This is the result for acetone

could you click on the icon with the red/green/yellow blocks (win95 defragment icon) and send a picture ? I'd like to check centering of the beam. If you can take a full screen image (full roi) with spinview that would also help.