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The idea of using a reference value was to compensate for small drifts in the amplitude of the applied sine wave.

I haven't test without it but when I designed the circuit I wanted to be safe and included as baseline.

The people from Strathclyde were kind enough to make a text version of the list at the bottom of the page here:

https://focalplane.biologists.com/2022/ ... f-systems/

The generic background correction method is not ideal for quantitative work.

The easiest is to export the raw spectrum in csv and, using Matlab or excel, remove a slanted line at the base of the peak. It works very well for isolated sharp peaks but it's more tricky for broader peaks.

There are two variables that can be used to define the noise of a spectrograph which are often mixed up. Here is a bit of clarity in that mix: Signal to Noise Ratio (SNR) which is technically how much noise is contained in every pixel of your data. To obtain it you typically take at least 10 spectra...

The issue with single mode fiber is that it's very difficult to couple the signal into it. And the big disadvantage of the larger core fiber is that you loose a lot of light. I remember that my boss tried to make an OpenRAMAN reproduction at the office except he decided to change the design to use a...

Considering the noise level in the spectrum shown, I would say that the approximation of ~5°C is not that far from reality.

I really have to take a moment to read that paper to get a better understanding of the experiment!

Is the setup at the VUB main campus in Etterbeek?

If so, maybe we can arrange a visit so that I have a look at your setup directly. Contact me via private message if you're interested.

My recommendation is to take the blank exactly like how you'd do the measurement but just switch the laser off - then switch the laser back on, wait for stabilization if required, and perform all your measurements. So I do it with the test tube in place :) And I even try to stay at the same position...

It's not going to be as-sensitive as an interferometer (which will react to people slamming doors etc.) but you might want to isolate it a bit in harsh environments. You have plenty of choices for that, but I'll focus on sorbothane feets: https://www.thorlabs.de/newgrouppage9.cfm?objectgroup_id=6421...

Something that I can add also is to take into account the fluorescence of the supporting medium. At the maximum exposure time of the camera, 32 seconds, I reach the fluorescence of water at ~50 mW so it makes no sense to use more power unless I plan to take shorter exposure time. That's if you want ...